Fluorescence:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Knockdown:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Staining:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Activity Assay:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Luciferase:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Control:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Immunofluorescence:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Expressing:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Binding Assay:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Activation Assay:Article Title: Membrane lipid composition and endocytosis modulate Wingless release from secreting cells
Article Snippet: They were distinguished based on the presence or absence of Myr-RFP.They were distinguished based on the presence or absence of Myr-RFP.. The following primary antibodies were used: mouse anti-Wingless (1:500, DSHB 4D4), rabbit anti-Wls (1:1000, generous gift from Konrad Basler, ( )), goat anti-GMAP (1:1000, DSHB GMAP), rabbit anti-HA (1:800, CST #3724), rat anti-DE-cadherin (1:50, DSHB DCAD2), mouse anti-Rab7 (1:10, DSHB Rab7-s), rat anti-Ci (1:100, DSHB 2A1-s) and mouse anti-Dlp (1:50, DSHB 13G8).. Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).Secondary antibodies used were anti-rabbit AlexaFluor488, anti-mouse Cy3, anti-rat AlexaFluor647 (1:400, Jackson ImmunoResearch); anti-rabbit CF405S, anti-rat CF405S (1:1000, Biotum); anti-rabbit AlexaFluor647, anti-mouse AlexaFluor405, anti-mouse AlexaFluor555, anti-mouse AlexaFluor647, anti-goat AlexaFluor647 (1:500, Invitrogen).
Article Title: Regeneration can take place across Drosophila compartments or segments with different Hox gene expression
Article Snippet: Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).Larvae were dissected in cold PBS and fixation was carried out in 4% PFA for 30 min. Dissection, fixation and staining of imaginal discs were done according to standard protocols ( ).. The antibodies used were mouse anti-Ubx 1:10 , rabbit anti-ßgalactosidase 1:2000 (Cappel), mouse anti-ßgalactosidase 1:100 (Sigma), mouse anti-Abd-B 1:125 (DSHB) , rat anti-Ci 1:200 (DSHB 2A1) , rabbit anti-Cad 1:200 (McDonald and Struhl, 1986), guinea-pig anti-Cad 1:200 (Kosman et al., 1988) and rabbit anti-Dcp1 1:200 (Cell Signaling, antibody #9578).. Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.Secondary antibodies were fluorescently labeled secondary antibodies (Jackson Immunoresearch) used at 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen (25)) 1:100; rabbit anti-Dcp1 (Cell Signalling, antibody #9578) 1:200; rabbit anti-Diap1 (a gift from H. Steller (39)) 1:2000.. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: The homeodomain-interacting protein kinase Hipk promotes apoptosis by stabilizing the active form of Dronc.
Article Snippet: They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; rabbit anti-Hipk (a gift from E. Verheyen; Ref. 25) 1:100; rabbit anti-Dcp1 (Cell Signaling, antibody #9578) 1:200; rabbit antiDiap1 (a gift from H. Steller; Ref.39) 1:2000.. AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.AR TI CL E IN P RE SS ARTICLE IN PRESS Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Dpp and Immune Response Pathways Factors Mediate Paracrine Induction of Senescent Cells in Drosophila
Article Snippet: They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).They were then washed 4 times in PBS, 0.3% Triton X-100 in the dark and mounted in Vectashield mounting medium (Vector Laboratories).. The following primary antibodies were used: rat anti-Ci (DSHB 2A1) 1:50; rabbit anti-PH3 (Cell Signal Technology) 1:100; mouse anti-Mmp1 (DSHB, a combination, 1:1:1, of 3B8D12, 3A6B4 and 5H7B11) 1:50; mouse anti-Wingless (DSHB 4D4) 1:50; mouse anti- b-galactosidase (DSHB 40-1a) 1:50; rabbit anti-pMad (1:100).. Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.Fluorescently labelled secondary antibodies (Molecular Probes Alexa-488, Alexa-555, Alexa-647, ThermoFisher Scientific) were used in a 1:200 dilution.
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production.
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/ tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
Article Title: Drosophila Dpp and BMP signaling directly regulates dpp transcription for optimal ligand production
Article Snippet: The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.The blocking and antibody incubation buffers were PBST (0.1% Triton X-100) with 5% fetal bovine serum.. Primary antibodies used were as follows: chicken anti-GFP (1:1000, Abcam, ab13970), rabbit anti-GFP (1:400, Invitrogen, A-11122), rabbit anti-pMad (1:400, CST, 41D10), rabbit anti-RFP/tdTMT (1:1000, Rockland Immunochemicals, 600-401-379), rat anti-Sal (1:400, lab-made), chicken anti-β-Galactosidase (1:1000, Abcam, ab134435), mouse anti-Ptc (1:50, DSHB, Apa1), rat anti-Ci (1:50, DSHB, 2A1), mouse anti-Engrailed (1:100, lab-made), goat anti-Distal-less (dF-20, 1:100, Santa Cruz, sc-15858) and mouse anti Flag [1: 1000, Sigma, F3165 (clone M2)].. Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey anti-rabbit Alexa Fluor 546 (A10040), donkey anti-rat Alexa Fluor 647 (A48272), goat anti-chicken Alexa Fluor 546 (A11040) and donkey anti-mouse Alexa Fluor 488 (A21202).Fluorescence-conjugated secondary antibodies were from Jackson ImmunoResearch Laboratories and were used at 1:400 dilution: donkey anti-chicken Alexa Fluor 488 (A11039), donkey anti-rabbit Alexa Fluor 488 (A21206), donkey..
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